Temporal focusing-based multiphoton excitation fluorescence microscopy system capable of tunable-wavelength excitation and excitation wavelength selection module thereof

Abstract

The invention provides a temporal focusing-based multiphoton excitation fluorescence microscopy system capable of tunable-wavelength excitation and an excitation wavelength selection module thereof. The temporal focusing-based multiphoton excitation fluorescence microscopy system comprises: an excitation light generating module for generating excitation light; an excitation wavelength selection module for generating reflected light having a predetermined output angle in accordance with the wavelength of the excitation light and generating detecting excitation light through a diffraction unit; and a fluorescent microscope. With the practice of the present invention, the wavelength of the excitation light can be changed by the excitation wavelength selection module in real time to excite different fluorophores with the corresponding maximum multiphoton absorption wavelengths, so as to obtain better fluorescent signals and improve the image quality of the temporal focusing-based multiphoton excitation fluorescence microscope system.