The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2019

Filed:

Feb. 28, 2012
Applicants:

Steen Hauge Matthiesen, Hilleroed, DK;

Søren Nielsen, Aalborg, DK;

Inventors:

Steen Hauge Matthiesen, Hilleroed, DK;

Søren Nielsen, Aalborg, DK;

Assignee:

DAKO DENMARK A/S, Glostrup, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); G01N 1/31 (2006.01); G01N 1/36 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); G01N 1/312 (2013.01); G01N 2001/366 (2013.01);
Abstract

The present application provides a two phase immiscible system for the pretreatment of embedded biological samples comprising placing at least one support having an embedded biological sample on its surface into a pretreatment container, adding to the pretreatment container at least one reagent forming a layer, adding a carrier composition to the pretreatment container, such that reagent forming layer is formed on the top of the carrier composition, and in an amount such that the at least one reagent forming layer contacts at least a portion of the embedded biological sample. Pretreatment of the embedded biological samples can include removal of embedding medium from embedded biological, target retrieval and enzyme blocking samples before staining histochemical analysis or other processes. The system also includes an apparatus and processes of automation of the pretreatment methods.


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