The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2019

Filed:

Mar. 04, 2015
Applicant:

East China University of Science and Technology, Shanghai, CN;

Inventors:

Jianhe Xu, Shanghai, CN;

Yujun Zhang, Shanghai, CN;

Gaowei Zheng, Shanghai, CN;

Jiang Pan, Shanghai, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/04 (2006.01); C12N 9/88 (2006.01); C12N 9/10 (2006.01); C12N 15/00 (2006.01); C12P 7/62 (2006.01); C12P 7/04 (2006.01); C12N 15/52 (2006.01); C12R 1/72 (2006.01); C12N 1/16 (2006.01); C12P 7/42 (2006.01); C12N 9/02 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 1/16 (2013.01); C12N 9/0036 (2013.01); C12N 15/81 (2013.01); C12P 7/42 (2013.01); C12R 1/72 (2013.01); C12Y 101/01184 (2013.01);
Abstract

Disclosed herein isCGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.


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