The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2019

Filed:

Jan. 11, 2017
Applicant:

Medipost Co., Ltd., Seoul, KR;

Inventors:

Yoon-Sun Yang, Seoul, KR;

Won Il Oh, Seoul, KR;

Sun Jae Kwon, Seoul, KR;

Mi Yeon Lee, Seoul, KR;

Hong Bae Jeon, Seoul, KR;

Assignee:

MEDIPOST CO., LTD, Seoul, KR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/0775 (2010.01); A61K 35/28 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0665 (2013.01); A61K 35/28 (2013.01); C12N 2500/02 (2013.01); C12N 2500/12 (2013.01); C12N 2500/14 (2013.01); C12N 2500/16 (2013.01); C12N 2500/84 (2013.01);
Abstract

Disclosed is a method for culturing mesenchymal stem cells, comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5% oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition, the mesenchymal stem cells prepared by the culturing method are effectively used not only as a safe cell therapeutic agent due to their lacking immunogenicity, but also as a cartilage regenerating medicine owing to their excellent secretion of cytokines.


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