The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 2019
Filed:
Mar. 03, 2014
Case Western Reserve University, Cleveland, OH (US);
Eric J. Arts, Cleveland, OH (US);
Alison Laing Wright, Cleveland, OH (US);
Kevin V. King, Cleveland, OH (US);
Miguel E. Quinones-Mateu, Cleveland, OH (US);
CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US);
Abstract
A method for determining HIV-1 co-receptor tropism in an HIV-infected patient includes preparing an HIV-1 envelope protein coding sequence from a sample, introducing the HIV-1 envelope protein coding sequence into a first expression construct by providing a plasmid expression vector including a near-full length HIV-1 genome having a yeast uracil biosynthesis gene in place of a HIV-1 env coding sequencing and replacing the yeast uracil biosynthesis gene with the HIV-1 envelope protein coding sequence prepared from the patient sample, and using the first expression construct in a cell to cell fusion assay to determine HIV-1 co-receptor tropism.