The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 14, 2019

Filed:

Dec. 31, 2015
Applicant:

Oakland University, Rochester, MI (US);

Inventor:

Xiangqun Zeng, Rochester Hills, MI (US);

Assignee:

Oakland University, Rochester, MI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/48 (2006.01); C12Q 1/18 (2006.01); G01N 27/416 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/18 (2013.01); G01N 27/4166 (2013.01);
Abstract

Real-time and end point determination of antibiotic effects are disclosed herein. In one example, a surface of a label free biosensor is exposed to a sample including a gram-negative bacteria. A frequency and/or a current of the biosensor is then allowed to reach a constant value. The surface of the biosensor is then exposed to an antibiotic. Using the biosensor, i) a frequency change versus time and a damping resistance versus time, or ii) a current versus voltage or the current versus time at a fixed potential, or iii) both i and ii are then measured. The frequency change versus time and the damping resistance versus time and/or the current versus voltage or the current versus time are correlated to determine an effect of the antibiotic on the gram-negative bacteria. Examples of the label free biosensors and methods for detecting gram-negative bacteria using the label free biosensors are also disclosed.


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