The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 14, 2019

Filed:

Jun. 16, 2014
Applicant:

The University of Notre Dame, Notre Dame, IN (US);

Inventors:

James Carter, Mishawaka, IN (US);

Malcolm J. Fraser, Jr., Granger, IN (US);

Assignee:

The University of Notre Dame, Notre Dame, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); G01N 33/569 (2006.01); C12N 15/113 (2010.01); G01N 33/53 (2006.01); G01N 33/58 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/70 (2006.01);
U.S. Cl.
CPC ...
C12N 15/11 (2013.01); C12N 15/1131 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/701 (2013.01); G01N 33/5308 (2013.01); G01N 33/56983 (2013.01); G01N 33/588 (2013.01); C12N 2310/127 (2013.01); C12N 2310/351 (2013.01); C12N 2310/3517 (2013.01); C12N 2320/10 (2013.01); C12N 2770/24122 (2013.01); G01N 2333/181 (2013.01); G01N 2333/185 (2013.01); Y02A 50/51 (2018.01); Y02A 50/53 (2018.01); Y02A 50/60 (2018.01);
Abstract

The present invention relates to DNAzymes (also known as deoxyribozymes, DNA enzymes, catalytic DNA, or DZ), which are conjugated to nanoparticles (NP) to facilitate the detection of nucleic acids. One aspect of the invention relates to compounds comprising DNAzymes conjugated to nanoparticles (DZ-NP), such as metallic or gold nanoparticles, and methods for their synthesis. Another aspect of the invention relates to methods of using the conjugated compounds to detect nucleic acids, such as genomic material or transcripts of infectious agents, such as viruses, exemplified by applications demonstrating visual detection of Flavivirus RNA molecules, such as dengue virus, or Alphavirus RNA molecules, such as chikungunya virus, in short time periods, using compositions comprising stable components.


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