The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 2019
Filed:
Feb. 18, 2015
Duke University, Durham, NC (US);
Emory University, Atlanta, GA (US);
Bryan R. Cullen, Durham, NC (US);
E. Matthew Kennedy, Durham, NC (US);
Hal P. Bogerd, Durham, NC (US);
Anand Kornepati, Durham, NC (US);
Adam Mefferd, Durham, NC (US);
Raymond F. Schinazi, Atlanta, GA (US);
Duke University, Durham, NC (US);
Emory University, Atlanta, GA (US);
The United States of America as Represented by the Department of Veterans Affairs, Washington, DC (US);
Abstract
Provided herein are recombinant constructs, vectors and expression cassettes including a first promoter which is suitably a tRNA promoter operably connected to a first polynucleotide encoding a first single guide RNA and a second promoter operably connected to a second polynucleotide encoding a Cas9 polypeptide. The first single guide RNA includes a first portion complementary to a strand of a target sequence of a DNA virus and a second portion capable of interacting with the Cas9 polypeptide. Also provided are codon optimizedderived Cas9 polynucleotides and polypeptides with nuclear localization signals and optionally an epitope tag. Also provided are constructs for production of sgRNAs including a tRNA. Methods of inhibiting viral replication, inhibiting expression of a target sequence from a virus or treating a viral infection or viral induced cancer using the compositions are also provided.