The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 30, 2019
Filed:
Mar. 24, 2011
Shinichi Yoshida, Takasago, JP;
Dai Murata, Takasago, JP;
Shunichi Taira, Takasago, JP;
Masayuki Takano, Takasago, JP;
Keita Iguchi, Takasago, JP;
Yoshiyuki Nakano, Takasago, JP;
Shinichi Yoshida, Takasago, JP;
Dai Murata, Takasago, JP;
Shunichi Taira, Takasago, JP;
Masayuki Takano, Takasago, JP;
Keita Iguchi, Takasago, JP;
Yoshiyuki Nakano, Takasago, JP;
KANEKA CORPORATION, Osaka, JP;
Abstract
An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.