The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 23, 2019
Filed:
Nov. 21, 2014
Centre National DE LA Recherche Scientifique, Paris, FR;
Institut Curie, Paris, FR;
Ecole Normale Superieure DE Paris, Paris, FR;
Universite Pierre ET Marie Curie, Paris, FR;
Ludovic Jullien, Arcueil, FR;
Thomas Le Saux, Paris, FR;
Arnaud Gautier, Paris, FR;
Vincent Croquette, Antony, FR;
Nath Sarang, New Delhi, IN;
Pencheng Wang, Saint Louis, MO (US);
Jérôme Querard, Montrouge, FR;
Samantha Albright, Boston, MA (US);
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris, FR;
INSTITUT CURIE, Paris, FR;
ECOLE NORMALE SUPERIEURE DE PARIS, Paris, FR;
UNIVERSITE PIERRE ET MARIE CURIE, Paris, FR;
Abstract
A method for detection of at least one reversibly photo-convertible fluorescent species, comprises the following steps: a) illumination of a sample comprising said or at least one of the reversibly photo-convertible fluorescent species by a periodically modulated illuminating light; and b) detection of fluorescent emission emitted by the sample thus illuminated; wherein the method further comprises the following step: c) extraction of the amplitude of the intensity component of the fluorescent emission exhibiting the same periodicity as the periodically modulated illuminating light and a phase quadrature with respect to the same; and wherein the mean intensity of the illuminating light and the modulation frequency of the same are chosen to maximize the amplitude of the intensity component of the fluorescent emission.