The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 23, 2019
Filed:
Nov. 03, 2015
Hitachi Chemical Company Ltd., Tokyo, JP;
Hitachi Chemical Co. America, Ltd., Cupertino, CA (US);
Beth Israel Deaconess Medical Center, Boston, MA (US);
Masato Mitsuhashi, Irvine, CA (US);
Alan C. Moss, Dover, MA (US);
Hitachi Chemical Company Ltd., Tokyo, JP;
Hitachi Chemical Co. America, Ltd., San Jose, CA (US);
Beth Israel Deaconess Medical Center, Boston, MA (US);
Abstract
The present disclosure relates to methods of collecting intestinal luminal fluid (ILF) exosomes and microvesicles (EMV) and isolating corresponding mRNA in order to diagnose and treat inflammatory bowel diseases (IBD). In particular, certain embodiments relate to the method of capturing EMV from ILF applied to a filter device that is capable of capturing EMV. Nucleic acids such as mRNA can be isolated from the EMV using an oligo(dT)-coated plate designed to accommodate the filter device and then used for further molecular analysis. Quantification of the collected nucleic acids may then be used in the diagnosis and/or treatment of IBD.