The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 23, 2019

Filed:

Jul. 31, 2013
Applicant:

Canon U.s. Life Sciences, Inc., Rockville, MD (US);

Inventors:

Hongye Liang, Clarksville, MD (US);

Kenton C. Hasson, Germantown, MD (US);

Assignee:

Canon U.S. Life Sciences, Inc., Rockville, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12Q 1/68 (2018.01); H04N 7/18 (2006.01); C12Q 1/686 (2018.01); B01L 7/00 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/68 (2013.01); B01L 3/5027 (2013.01); B01L 7/52 (2013.01); C12Q 1/686 (2013.01); G01N 21/6456 (2013.01); H04N 7/183 (2013.01); B01L 3/502715 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/049 (2013.01);
Abstract

This invention relates to systems and methods for imaging sample materials within a microfluidic device during an assay reaction process. In accordance with certain aspects of the invention, images are formed with a pixel array and a region of interest ('ROI') is defined within the pixel array. Image values, such as fluorescent intensity, can be computed as averages of individual pixel values within the ROI. Where the ROI is subject to non-uniform conditions, such as non-uniform heating, the ROI can be divided into sub-ROIs which are sufficiently small that the condition is uniform within the sub-ROI.


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