The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 23, 2019
Filed:
Jan. 09, 2015
Applicant:
Synthon Biopharmaceuticals B.v., Nijmegen, NL;
Inventors:
Guy De Roo, Nijmegen, NL;
Ruud Martin Verstegen, Nijmegen, NL;
Rudy Gerardus Elisabeth Coumans, Nijmegen, NL;
Assignee:
Synthon Biopharmaceuticals B.V., Nijmegen, NL;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/32 (2006.01); A61K 47/68 (2017.01);
U.S. Cl.
CPC ...
C07K 16/32 (2013.01); A61K 47/6803 (2017.08); A61K 47/6851 (2017.08); A61K 47/6889 (2017.08);
Abstract
The present invention relates to a method for purifying a mixture of cysteine-linked antibody-drug conjugates, wherein the amount of non-conjugated antibody is in the range of 0-40% by weight, using hydrophobic interaction chromatography (HIC). The mixture is loaded onto a preparative HIC column using a 0.2-1.5 M aqueous salt solution, in which non-conjugated antibody is collected in a flow-through fraction, followed by elution of a purified mixture of cysteine-linked antibody-drug conjugates using a 0-100 mM aqueous salt solution.