The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 16, 2019

Filed:

May. 29, 2015
Applicants:

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, CN;

Tianjin Sinonocy Biological Technology Co., Ltd., Tianjin, CN;

Inventors:

Wenxia Jiang, Tianjin, CN;

Qi Liu, Tianjin, CN;

Weiya Zhang, Tianjin, CN;

Baoliang Mei, Tianjin, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/10 (2006.01); C12N 1/14 (2006.01); C12P 13/04 (2006.01); C12R 1/645 (2006.01);
U.S. Cl.
CPC ...
C12P 17/10 (2013.01); C12N 1/14 (2013.01); C12P 13/04 (2013.01); C12N 2500/32 (2013.01); C12N 2500/35 (2013.01); C12R 1/645 (2013.01);
Abstract

The present disclosure relates to an improved method for producing ergothioneine, comprising the steps of: (a) inoculatingstrain CGMCC No.6232 into a seed medium, and culturing it to prepare a seed liquor, wherein the seed medium uses soybean cake powder as nitrogen source; and (b) inoculating the seed liquor into a fermentation basal medium, and then culturing it to obtain a fermentation broth ofmycelia. Further, any one or more members selected from NHCl, NHNO, NaCl, polyethylene glycol, folic acid, vitamin B1 (VB1), indolebutyric acid, citric acid, pyruvic acid, arginine, lysine, leucine, aspartic acid, glutamic acid, betaine, histidine, cysteine, methionine, tween, span, chitosan, Fluconazole, Miconazole, Ketoconazole, ethylenediaminetetraacetic acid (EDTA), isopropyl alcohol and dimethyl sulfoxide are added into the fermentation basal medium.


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