The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 16, 2019
Filed:
Mar. 26, 2015
Applicant:
The Johns Hopkins University, Baltimore, MD (US);
Inventors:
David K. Karig, Columbia, MD (US);
Joshua T. Wolfe, Bethesda, MD (US);
Assignee:
The Johns Hopkins University, Baltimore, MD (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1058 (2013.01); C12N 15/1075 (2013.01);
Abstract
A method of directed evolution screening includes selecting a protein expression platform for an evolution target, expressing a key rupture gene configured to trigger droplet rupture, developing gene regulatory circuits to control expression of the key rupture gene as a function of performance of the evolution target, encapsulating expression components in droplets, and triggering droplet rupture by expressing a rupture agent from the key rupture gene.