The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 16, 2019
Filed:
Feb. 12, 2018
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Christopher H. Taron, Essex, MA (US);
Saulius Vainauskas, Newburyport, MA (US);
Xiaofeng Shi, Beverly, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/26 (2006.01); C07H 5/06 (2006.01); C12N 9/24 (2006.01); C07K 1/13 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2402 (2013.01); C07H 5/06 (2013.01); C07K 1/13 (2013.01); C12P 21/005 (2013.01); C12Y 302/01023 (2013.01); C12Y 302/01111 (2013.01); C12Y 302/01127 (2013.01);
Abstract
Provided herein is an α-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The α-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the α-fucosidase are provided, as well as a method for analyzing glycoproteins. The α-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.