The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 16, 2019
Filed:
May. 13, 2014
Medimmune Limited, Cambridge, GB;
Medimmune, Llc, Gaithersburg, MD (US);
David Edwin Gruber, Cambridge, GB;
Richard Edward Turner, Cambridge, GB;
Jared Samuel Bee, Gaithersburg, MD (US);
Christopher Douglas Afdahl, Gaithersburg, MD (US);
Liu Tie, Gaithersburg, MD (US);
MedImmune, LLC, Gaithersburg, MD (US);
Medimmune Limited, Cambridge, GB;
Abstract
Described herein is a method for separating a recombinantly produced polypeptide from host cell protein. The method includes a step of loading a clarified cell culture supernatant that includes the recombinantly produced polypeptide and the HCP onto a Protein A chromatography column and washing the Protein A chromatography column with a wash buffer comprising a fatty acid having a chain length of at least about 6 carbon atoms, or a fatty acid salt thereof to remove HCP and then recovering the recombinantly produced polypeptide.