The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 16, 2019

Filed:

Nov. 30, 2016
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Kevin D. Ness, Pleasanton, CA (US);

Benjamin J. Hindson, Livermore, CA (US);

Billy W. Colston, Jr., San Ramon, CA (US);

Donald A. Masquelier, Tracy, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01F 3/08 (2006.01); B01F 13/00 (2006.01); B01L 3/02 (2006.01); B01L 7/00 (2006.01); G01N 21/3563 (2014.01); C12Q 1/686 (2018.01); G01N 21/64 (2006.01); B01F 15/00 (2006.01); B29C 45/00 (2006.01); G01N 21/49 (2006.01); B29L 31/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502784 (2013.01); B01F 3/0807 (2013.01); B01F 13/0062 (2013.01); B01F 15/00922 (2013.01); B01L 3/0241 (2013.01); B01L 3/502715 (2013.01); B01L 7/525 (2013.01); B29C 45/006 (2013.01); B29C 45/0053 (2013.01); C12Q 1/686 (2013.01); G01N 21/3563 (2013.01); G01N 21/49 (2013.01); G01N 21/6428 (2013.01); G01N 21/6486 (2013.01); B01F 2003/0834 (2013.01); B01F 2003/0842 (2013.01); B01F 2215/0037 (2013.01); B01L 7/52 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/12 (2013.01); B01L 2300/041 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); B29C 2045/0079 (2013.01); B29L 2031/752 (2013.01); G01N 2021/6439 (2013.01); Y02A 90/26 (2018.01);
Abstract

Methods of making a droplet-generating device. In an exemplary method, an upper member is injection molded. The upper member includes a bottom surface and also includes a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface. The upper member has a plurality of openings each extending completely through the upper member from the bottom surface and creating a side wall region of a sample well, a carrier well, and a droplet well. A cover layer is attached to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel. The microfluidic channels meet one another to create a droplet-generation region. The sample well, the carrier well, and the droplet well are connected to the droplet-generation region via the first, second, and third microfluidic channels, respectively.


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