The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 09, 2019
Filed:
Jun. 01, 2017
Applicant:
Medimmune Limited, Cambridge, GB;
Inventors:
Kenneth H. Grabstein, Mercer Island, WA (US);
Michael Van Brunt, Covington, WA (US);
Marcello Marelli, Seattle, WA (US);
William Brady, San Diego, CA (US);
Jeffrey C. Johnson, Flemington, NJ (US);
Assignee:
MEDIMMUNE LIMITED, Cambridge, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/02 (2006.01); A61K 47/68 (2017.01); C07C 271/12 (2006.01); C07C 271/20 (2006.01); C07C 271/22 (2006.01); C07C 233/49 (2006.01); C07C 233/56 (2006.01); C07C 247/04 (2006.01); C07C 17/08 (2006.01); C07K 16/24 (2006.01); C07K 16/32 (2006.01); C07C 235/74 (2006.01); C07K 16/46 (2006.01); C07K 17/08 (2006.01); C07K 14/435 (2006.01); C07K 16/28 (2006.01); C07K 16/40 (2006.01); C12N 9/00 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); A61K 47/6851 (2017.08); C07C 233/49 (2013.01); C07C 233/56 (2013.01); C07C 235/74 (2013.01); C07C 247/04 (2013.01); C07C 271/12 (2013.01); C07C 271/20 (2013.01); C07C 271/22 (2013.01); C07K 14/43595 (2013.01); C07K 16/244 (2013.01); C07K 16/248 (2013.01); C07K 16/2863 (2013.01); C07K 16/32 (2013.01); C07K 16/40 (2013.01); C07K 16/46 (2013.01); C07K 17/08 (2013.01); C12N 9/93 (2013.01); C12N 15/85 (2013.01); C07K 2317/14 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/40 (2013.01); C07K 2317/51 (2013.01); C12Y 301/01025 (2013.01); C12Y 601/01 (2013.01); C12Y 601/01025 (2013.01);
Abstract
There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.