The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 09, 2019

Filed:

Mar. 23, 2017
Applicant:

Alliance for Sustainable Energy, Llc, Golden, CO (US);

Inventors:

Gregg Tyler Beckham, Golden, CO (US);

Christopher W. Johnson, Denver, CO (US);

Derek R. Vardon, Lakewood, CO (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/44 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/18 (2006.01); C12N 9/90 (2006.01); C12N 9/88 (2006.01);
U.S. Cl.
CPC ...
C12P 7/44 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/0069 (2013.01); C12N 9/13 (2013.01); C12N 9/18 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12Y 101/01312 (2013.01); C12Y 102/01085 (2015.07); C12Y 113/11001 (2013.01); C12Y 113/11003 (2013.01); C12Y 113/11008 (2013.01); C12Y 208/03006 (2013.01); C12Y 301/01024 (2013.01); C12Y 301/01057 (2013.01); C12Y 307/01009 (2013.01); C12Y 401/01044 (2013.01); C12Y 401/01045 (2013.01); C12Y 401/01077 (2013.01); C12Y 402/01083 (2013.01); C12Y 503/02 (2013.01); C12Y 503/02006 (2015.07); C12Y 503/03004 (2013.01); C12Y 505/01001 (2013.01); C12Y 505/01002 (2013.01);
Abstract

An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.


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