The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 09, 2019

Filed:

Apr. 27, 2017
Applicant:

Fenwal, Inc., Lake Zurich, IL (US);

Inventors:

Christopher J. Wegener, Libertyville, IL (US);

Bret M. Olson, Chicago, IL (US);

Alaina Schlinker, Chicago, IL (US);

Steven Binninger, Evanston, IL (US);

Avnie A. Kadakia, Chicago, IL (US);

Assignee:

Fenwal, Inc., Lake Zurich, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61M 1/34 (2006.01); A61M 1/26 (2006.01); A61M 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/12 (2006.01); C12Q 1/24 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
A61M 1/3496 (2013.01); A61M 1/265 (2014.02); A61M 1/362 (2014.02); C12M 23/28 (2013.01); C12M 25/02 (2013.01); C12M 27/02 (2013.01); C12M 29/00 (2013.01); C12M 29/04 (2013.01); C12Q 1/24 (2013.01); G01N 35/1016 (2013.01); G01N 2035/00465 (2013.01);
Abstract

A method for automated processing of a cellular product comprising target substrate cells, the method comprising providing a separation apparatus configured to associate with a disposable sterile circuit comprising a separator in communication with the cellular product. The apparatus and disposable sterile circuit are configured to remove platelets from the cellular product to form a platelet-depleted cellular product, resuspend the platelet-depleted cellular product in media to form a resuspended platelet-depleted cellular product, receive an agent having an association with the target substrate cells of the resuspended platelet-depleted cellular product, incubate the agent with the target substrate cells over a period sufficient for the agent to bind with and/or enter the target substrate cells to form a first mixture comprising agent-target substrate cell complexes, unbound/unassociated agent, and non-target substrate cells, and remove unbound/unassociated agent to form a second mixture comprising the agent-target substrate cell complexes and non-target substrate cells.


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