The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 09, 2019

Filed:

Sep. 26, 2014
Applicant:

Kymab Limited;

Inventor:

Jasper Clube, Cambridge, GB;

Assignee:

Kymab Limited, Cambridge, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C12N 15/06 (2006.01); C12N 15/87 (2006.01); A61K 39/395 (2006.01); C07H 21/04 (2006.01); C07K 16/46 (2006.01); C12N 15/85 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0278 (2013.01); C07K 16/00 (2013.01); C07K 16/461 (2013.01); C07K 16/462 (2013.01); C12N 15/8509 (2013.01); A01K 2207/15 (2013.01); A01K 2217/072 (2013.01); A01K 2217/15 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); C07K 2317/92 (2013.01);
Abstract

The present invention relates to humanisation of antibodies in vivo. The invention provides non-human vertebrates, cells, populations and methods useful for humanising chimaeric antibodies in vivo. Using the present invention it is possible straightforwardly and rapidly to obtain antigen-specific antibodies that are fully human (ie, comprising human variable and constant regions) and have undergone recombination, junctional diversification, affinity maturation and isotype switching in vivo in a non-human vertebrate system. Furthermore, such antibodies are humanised (eg, totally human)—and selected—totally in vivo, and as such the present invention harnesses in vivo filtering for expressibility, affinity and biophysical characteristics in the context of the desired human variable and constant region pairings. This is avoids problems of down-grading antibody characteristics when humanising the constant region of chimaeric antibodies in vitro.


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