The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 02, 2019
Filed:
Jul. 30, 2014
Sphere Fluidics Limited, Babraham Cambridge, Cambridgeshire, GB;
Clive A. Smith, Babraham Cambridge, GB;
Xin Li, Babraham Cambridge, GB;
Karine Enesa, Babraham Cambridge, GB;
Xin Liu, Babraham Cambridge, GB;
Frank F. Craig, Babraham Cambridge, GB;
Graeme Whyte, Uttenreuth, DE;
SPHERE FLUIDICS LIMITED, Babraham, Cambridge, Cambridgeshire, GB;
Abstract
A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.