The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 02, 2019

Filed:

Oct. 04, 2017
Applicant:

Omniome, Inc., San Diego, CA (US);

Inventors:

Kandaswamy Vijayan, San Diego, CA (US);

Corey M. Dambacher, La Jolla, CA (US);

Eugene Tu, San Diego, CA (US);

Mark A. Bernard, Poway, CA (US);

Joseph Rokicki, San Diego, CA (US);

Kerry Wilson, La Jolla, CA (US);

Assignee:

OMNIOME, INC., San Diego, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); G01N 33/50 (2006.01); C12Q 1/6874 (2018.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 15/1068 (2013.01); C12P 19/34 (2013.01); C12Q 1/6874 (2013.01); G01N 33/50 (2013.01); C12Q 2521/101 (2013.01);
Abstract

Provided are compositions, methods and systems for determining the sequence of a template nucleic acid using a polymerase-based, sequencing-by-binding procedure. An examination step involves monitoring the interaction between a polymerase and template nucleic acid in the presence of one or more nucleotides. Identity of the next correct nucleotide in the sequence is determined without incorporation of any nucleotide into the structure of the primer by formation of a phosphodiester bond. An optional incorporation step can be used after the examination step to extend the primer by one or more nucleotides, thereby incrementing the template nucleotides that can be examined in a subsequent examination step. The sequencing-by-binding procedure does not require the use of labeled nucleotides or polymerases, but optionally can employ these reagents.


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