The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 26, 2019

Filed:

Dec. 22, 2016
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Amy L. Hiddessen, Tracy, CA (US);

Erin R. Chia, Walnut Creek, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); B01F 13/00 (2006.01); B01F 5/06 (2006.01); G01N 1/38 (2006.01); G01N 21/64 (2006.01); C12Q 1/6816 (2018.01); B01F 5/00 (2006.01); B01F 9/00 (2006.01); B01F 11/00 (2006.01); C12Q 1/6806 (2018.01); B01L 3/00 (2006.01); G01N 1/28 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6816 (2013.01); B01F 5/0085 (2013.01); B01F 5/0647 (2013.01); B01F 9/00 (2013.01); B01F 11/00 (2013.01); B01F 13/0062 (2013.01); B01F 13/0072 (2013.01); B01L 3/502784 (2013.01); C12Q 1/6806 (2013.01); G01N 1/38 (2013.01); G01N 21/645 (2013.01); G01N 21/6428 (2013.01); B01F 2215/0037 (2013.01); B01L 3/52 (2013.01); B01L 2200/148 (2013.01); B01L 2200/185 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2300/1861 (2013.01); G01N 2001/2893 (2013.01); G01N 2021/6439 (2013.01); G01N 2201/127 (2013.01);
Abstract

Method of detecting a signal from droplets for instrument calibration. In the method, a calibration standard for a droplet detection instrument may be received. The calibration standard may have been shipped at least one kilometer when received. The calibration standard may include a mixture of first droplets and second droplets. Each of the first and second droplets may be encapsulated by an immiscible carrier liquid and may have a stabilizing droplet skin that is formed by heating and that includes a skin-forming protein. Each the first droplets may contain a fluorescent dye, and each of the second droplets may contain a fluorescent dye. A fluorescence signal may be detected from a plurality of the first and second droplets with a droplet detection instrument. The fluorescence signal may be stronger for the first droplets than the second droplets.


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