The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 26, 2019
Filed:
Oct. 11, 2012
The Royal Veterinary College, London, GB;
THE ROYAL VETERINARY COLLEGE, London, GB;
Abstract
A method for promoting entry of an agent (introduced agent) into a cell, the method comprising the step of complexing the introduced agent in the presence of an entry-promoting agent and then exposing to cells, wherein the entry-promoting agent comprises a linear and/or branched or cyclic polymonoguanide/polyguanidine, polybiguanide, analogue or derivative thereof according to the following Formula 1a & b. The method also provides a means for formation of nanoparticles formed between the entry promoting agent and the introduced agent. wherein: 'n', refers to number of repeating units in the polymer, and n can vary from 2 to 1000, for example from 2 or 5 to 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, 700, 800 or 900; Gand Gindependently represent a cationic group comprising biguanide or guanidine, wherein L, and Lare directly joined to a Nitrogen atom of the guanide; Land Lare linking groups between the Gand Gcationic groups in the polymer and independently represent an aliphatic group containing C-Ccarbon atoms, for example an alkyl group such as methylene, ethylene, propylene, C, C, C, C, C, Cor C; C-C, -C, -C, -C, -C-C-C, -C, -C0, -C-, -C, -C, -Cor -C, alkyl; or a C-C(for example C, C, C, C, C, C, C, C, Cor C; C-C, -C, -C, -C, -C-C, - C, -C, -C, -C, -C0, -C, -Co or -C), cycloaliphatic, heterocyclic, aromatic, aryl, alkylaryl, arylalkyl, or oxyalkylene radical; or a polyalkylene radical optionally interrupted by one or more, preferably one, oxygen, nitrogen or sulphur atoms, functional groups or saturated or unsaturated cyclic moiety; N and Gare optional end groups; X can be either present or absent; L, Land X are linking groups between the Gand Gcationic groups in the polymer and independently represent an aliphatic group containing C-Ccarbon atoms, for example an alkyl group such as methylene, ethylene, propylene, C, C, C, C, C, Cor C; C-C, -C, -C, -C, -C-C, -C, -C, -C, -C, -C, -C, -Cor -C, alkyl; or Land Land X can independently be C-C(for example C, C, C, C, C, C, C, C, Cor C; C-C, -C, -C, -C, -C-C, -C, -C, -C, -C, -C, -C, -Cor -C), cycloaliphatic, heterocyclic, aromatic, aryl, alkylaryl, arylalkyl, oxyalkylene radicals; or a polyalkylene radical optionally interrupted by one or more, preferably one, oxygen, nitrogen or sulphur atoms, functional groups as well as saturated or unsaturated cyclic moiety; 'G' and “G” are cationic moieties and can be same or different, and at least one of them is a biguanidine moiety or carbamoylguanidine, and the other moiety may be biguanidine or carbamoylguanidine or amine; and cationic moieties Gand Gdo not contain single guanidine groups. The entry-promoting agent may comprise homogeneous or heterogeneous mixture of one or more of agents arising from formula 1 a and b, for example polyhexamethylene biguanide (PHMB), polyhexamethylene monoguanide (PHMG), polyethylene biguanide (PEB), polytetramethylene biguanide (PTMB), polyethylene hexamethylene biguanide (PEHMB), polymethylene biguanides (PMB), poly(allylbiguanidnio-co-allylamine), poly(N-vinylbiguanide), polyallybiguanide.