Monohydroxyphenyl metabolite urine detection reagent and preparation method thereof

Abstract

A reagent for detection of urine monophenolic metabolites and a method for preparing the reagent are disclosed, in which the monophenolic metabolites, for example, tyrosine, p-hydroxyphenyl alanine, tryptophan and 5-hydroxyindoleacetic acid can serve as tumor markers. The reagent for detection of urine monophenolic metabolites is an aqueous solution containing nitric acid, sulfuric acid, mercuric sulfate, mercurous nitrate, nickel nitrate, phosphomolybdic acid and cobalt sulfate. The preparation method includes preparation of solutions A, B, C, D and E, and mixing. The subject matter allows easy availability of raw materials, low cost, a simple preparation process, obtainment of reagents with stable performance which offer the advantages including high versatility, high sensitivity and good specificity when used in cancer detection, a simple detection process, a short detection cycle and easy determination, and is particularly suitable for large population screening, assistance in clinical cancer diagnosis and dynamic follow-up.