The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 19, 2019
Filed:
Sep. 26, 2013
Applicant:
Cepheid, Sunnyvale, CA (US);
Inventors:
Edwin Wei-Lung Lai, Santa Clara, CA (US);
Reuel Van Atta, Sunnyvale, CA (US);
Kenneth E. Ho, Sunnyvale, CA (US);
Assignee:
CEPHEID, Sunnyvale, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6886 (2018.01); G06F 19/20 (2011.01); G01N 1/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6886 (2013.01); G06F 19/20 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); G01N 1/06 (2013.01);
Abstract
Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.