The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 12, 2019
Filed:
Oct. 29, 2014
Applicant:
Region Syddanmark, Vejle, DK;
Inventor:
Niels Pallisgaard, Odense M, DK;
Assignee:
REGION SYDDANMARK, Vejle, DK;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6886 (2018.01); C12Q 1/6881 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C12Q 1/6881 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01);
Abstract
Methods and kits for qualifying the analysis of cell free DNA in e.g. plasma and serum samples are provided, based on the identification of contaminating DNA from B lymphocytes. Quantitative PCR (qPCR) can be used to detecting or determining the level of clonally rearranged immunoglobulin heavy-chain (IGH) genes, immunoglobulin kappa chain (IGK) genes, or immunoglobulin lambda-chain (IGL) genes, or a combination of any thereof. Samples identified as containing contaminating DNA can thus be identified and excluded or corrected, improving the accuracy of cf DNA determinations as a diagnostic, prognostic and treatment monitoring tool.