The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 12, 2019

Filed:

Apr. 27, 2018
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Matthew T. O'Keefe, Saratoga, CA (US);

Pamela K. Foreman, Los Altos, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12Q 1/686 (2018.01); B01F 13/00 (2006.01); B01F 15/02 (2006.01); B01L 3/00 (2006.01); H01J 49/04 (2006.01); C12Q 1/6883 (2018.01); C40B 40/06 (2006.01); C40B 60/14 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); B01F 13/0071 (2013.01); B01F 13/0084 (2013.01); B01F 15/0232 (2013.01); B01L 3/5025 (2013.01); B01L 3/50857 (2013.01); C12Q 1/6883 (2013.01); H01J 49/0418 (2013.01); B01F 13/0059 (2013.01); B01F 15/0201 (2013.01); B01F 2215/0431 (2013.01); B01J 2219/0072 (2013.01); B01J 2219/0074 (2013.01); B01J 2219/00286 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00722 (2013.01); B01L 3/5088 (2013.01); B01L 2200/0642 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/10 (2013.01); B01L 2300/165 (2013.01); B01L 2400/0688 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/172 (2013.01); C40B 40/06 (2013.01); C40B 60/14 (2013.01); Y02P 20/582 (2015.11);
Abstract

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.


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