The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 12, 2019

Filed:

Apr. 07, 2015
Applicant:

Bayer Healthcare Llc, Whippany, NJ (US);

Inventors:

Yuval Shimoni, Berkeley, CA (US);

Volker Möhrle, Köln, DE;

Assignee:

Bayer HealthCare LLC, Whippany, NJ (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/07 (2010.01); C12N 5/02 (2006.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0037 (2013.01); C12N 5/0018 (2013.01); C12N 5/0031 (2013.01); C12N 2500/05 (2013.01); C12N 2500/14 (2013.01); C12N 2500/22 (2013.01); C12N 2500/24 (2013.01); C12N 2500/32 (2013.01); C12N 2500/34 (2013.01); C12N 2500/42 (2013.01); C12N 2500/44 (2013.01); C12N 2500/46 (2013.01); C12N 2500/60 (2013.01); C12N 2501/10 (2013.01);
Abstract

The present invention provides a compounded cell culture medium powder formulation comprising: a basal medium powder and a cell culture media supplement, wherein the cell culture media supplement comprises and one or more salts; one or more growth factors; one or more inorganic ions; an amino acid supplement comprising one or more of asparagine, glutamine, histidine, and serine; one or more buffers; and one or more anti-foaming agents. The invention further provides methods of making a compounded cell culture medium powder formulation methods of making a cell culture medium for growing mammalian cells and methods of producing a protein of interest by culturing cells in the cell culture medium and isolating the protein of interest.


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