The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 05, 2019

Filed:

Jun. 30, 2015
Applicant:

Dana-farber Cancer Institute, Inc., Boston, MA (US);

Inventors:

Eugen Dhimolea, Malden, MA (US);

Constantine Mitsiades, Boston, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/00 (2006.01); C12M 1/32 (2006.01); C12M 1/12 (2006.01); C12M 1/00 (2006.01);
U.S. Cl.
CPC ...
C12M 23/12 (2013.01); C12M 21/08 (2013.01); C12M 23/06 (2013.01); C12M 25/04 (2013.01); C12M 25/14 (2013.01); C12M 29/10 (2013.01);
Abstract

Systems, devices, and methods for generating, culturing, and using magnetically-controlled three-dimensional (3D) tissues are described. A magnetically-enabled transwell system for immobilizing and supplying perfusion to a 3D mini-tissue includes a cell culture vessel with at least a first chamber and a second chamber, the mini-tissue being disposed in the first chamber, and a perfusion mechanism defining an elongate perfusion channel with a proximal end in the first chamber and a distal end in fluid communication with the second chamber, with a magnetic element configured to immobilize a magnetically-controlled 3D mini-tissue such that the proximal end is in fluid communication with basolateral space of the immobilized mini-tissue.


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