The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 05, 2019

Filed:

Oct. 09, 2014
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Kang Zhang, San Diego, CA (US);

Jiagang Zhao, San Diego, CA (US);

Adah Almutairi, La Jolla, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); A61L 27/38 (2006.01); C12N 5/0797 (2010.01); A61K 35/30 (2015.01); C12N 5/0793 (2010.01); C12N 5/079 (2010.01); A61L 27/52 (2006.01); A61K 35/545 (2015.01); A61L 27/24 (2006.01); A61L 27/26 (2006.01); A61L 27/54 (2006.01); A61K 9/00 (2006.01);
U.S. Cl.
CPC ...
A61L 27/3834 (2013.01); A61K 9/0046 (2013.01); A61K 35/30 (2013.01); A61K 35/545 (2013.01); A61L 27/24 (2013.01); A61L 27/26 (2013.01); A61L 27/3804 (2013.01); A61L 27/3839 (2013.01); A61L 27/52 (2013.01); A61L 27/54 (2013.01); C12N 5/062 (2013.01); C12N 5/0621 (2013.01); C12N 5/0623 (2013.01); A61L 2300/64 (2013.01); A61L 2430/16 (2013.01); C12N 2500/33 (2013.01); C12N 2500/38 (2013.01); C12N 2500/90 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/165 (2013.01); C12N 2501/39 (2013.01); C12N 2501/395 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01);
Abstract

The invention provides an in vitro method for producing isolated mammalian primitive retinal stem cells (pRSCs) comprising: (a) culturing isolated embryonic stem cells (ESCs) from a mammal in a cell culture medium that is free of feeder cells, feeder-conditioned medium or serum so as to produce and grow a culture of the isolated ESCs; and (b) contacting the culture of the isolated ESCs so grown with one or more of an inhibitor for Wnt or TGF-β/BMP signaling so as to differentiate the isolated ESCs of (a) into primitive retinal stem cells thereby producing isolated mammalian pRSCs.


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