The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 26, 2019

Filed:

Jun. 05, 2017
Applicant:

The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US);

Inventors:

Jeffrey T. Borenstein, Newton, MA (US);

Joseph L. Charest, Cambridge, MA (US);

Christopher M. DiBiasio, Stoughton, MA (US);

Dorit Berlin, Lexington, MA (US);

Jenna Balestrini, Boston, MA (US);

Jose A. Santos, Westwood, MA (US);

Vishal Tandon, Roxbury Crossing, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/42 (2006.01); C12N 15/87 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/0783 (2010.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/87 (2013.01); B01L 3/502746 (2013.01); C12M 23/40 (2013.01); C12M 25/00 (2013.01); C12M 27/18 (2013.01); C12M 29/04 (2013.01); C12M 35/08 (2013.01); C12N 5/0636 (2013.01); B01L 3/502761 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0877 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); C12N 2510/00 (2013.01);
Abstract

Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.


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