The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 26, 2019
Filed:
Sep. 02, 2016
Applicant:
Octapharma Ag, Lachen, CH;
Inventors:
Assignee:
OCTAPHARMA AG, Lachen, CH;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 1/16 (2006.01); C07K 1/36 (2006.01); B01D 15/32 (2006.01); B01D 15/34 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); C07K 14/52 (2006.01); C07K 14/475 (2006.01); C07K 14/535 (2006.01); C07K 14/575 (2006.01);
U.S. Cl.
CPC ...
C07K 14/535 (2013.01); B01D 15/34 (2013.01); B01D 15/362 (2013.01); B01D 15/3828 (2013.01); B01D 15/3847 (2013.01); C07K 1/165 (2013.01); C07K 1/36 (2013.01); C07K 14/475 (2013.01); C07K 14/52 (2013.01); C07K 14/575 (2013.01);
Abstract
A method of purifying a Growth Factor Protein in a purification sequence employing chromatography is provided. The method comprises performing at least one chromatography step using a multimodal resin, binding the Growth Factor Protein to the multimodal resin at a pH from 4 to 6.2, and eluting the Growth Factor Protein at a pH in the range of from 5.5 to 6.5. The elution of Growth Factor Protein is improved by addition of arginine and/or NaCl in the eluting buffer. Optionally the multimodal resin step is followed by a yeast derived affinity ligand resin step, which results in a purity of the product greater than 90%.