The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 12, 2019

Filed:

Sep. 03, 2015
Applicant:

Provivi, Inc., Santa Monica, CA (US);

Inventors:

Pedro Coelho, Santa Monica, CA (US);

Mike M. Y. Chen, Santa Monica, CA (US);

Peter Meinhold, Santa Monica, CA (US);

Catherine Mee-Hie Cho, Santa Monica, CA (US);

Vu Bui, Santa Monica, CA (US);

Thomas Heel, Santa Monica, CA (US);

Assignee:

PROVIVI, INC., Santa Monica, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/04 (2006.01); C12P 33/06 (2006.01); C07C 29/32 (2006.01); C07C 67/14 (2006.01); C07C 45/29 (2006.01); C07C 51/235 (2006.01); C07C 1/34 (2006.01); C07C 2/86 (2006.01); C07C 5/09 (2006.01); C07C 6/04 (2006.01); C12P 5/02 (2006.01); C12P 7/64 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01);
U.S. Cl.
CPC ...
C12P 7/04 (2013.01); C07C 1/34 (2013.01); C07C 2/861 (2013.01); C07C 5/09 (2013.01); C07C 6/04 (2013.01); C07C 29/32 (2013.01); C07C 45/29 (2013.01); C07C 51/235 (2013.01); C07C 67/14 (2013.01); C12P 5/026 (2013.01); C12P 7/6418 (2013.01); C07C 2523/44 (2013.01); C07C 2531/22 (2013.01); C07C 2531/24 (2013.01);
Abstract

In certain aspects, the present invention provides methods for producing terminally hydroxylated alkenes and alkynes by contacting an unsaturated or saturated hydrocarbon substrate with a hydroxylase enzyme. Exemplary terminal hydroxylases useful for carrying out the methods of the invention exhibit strong selectivity towards one terminal carbon of a hydrocarbon substrate and include, but are not limited to, non-heme diiron alkane monooxygenases, cytochromes P450 (e.g., cytochromes P450 of the CYP52 and CYP153 family), as well as long chain alkane hydroxylases. In some embodiments, the terminally hydroxylated alkene or alkyne is further converted to a terminal alkenal. In certain embodiments, terminally hydroxylated alkenes and alkynes are useful as insect pheromones which modify insect behavior. In other embodiments, terminally hydroxylated alkenes and alkynes are useful intermediates for producing pheromones via acetylation or oxidation of the alcohol moiety.


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