The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 05, 2019

Filed:

Nov. 08, 2011
Applicants:

Detlev Suckau, Grasberg, DE;

Martin Schürenberg, Tarmstedt, DE;

Rainer Paape, Martfeld, DE;

Christine Lübbert, Berlin, DE;

Inventors:

Detlev Suckau, Grasberg, DE;

Martin Schürenberg, Tarmstedt, DE;

Rainer Paape, Martfeld, DE;

Christine Lübbert, Berlin, DE;

Assignee:

Other;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 30/72 (2006.01); G01N 33/68 (2006.01); G01N 23/2258 (2018.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); G01N 2400/00 (2013.01);
Abstract

A method for the identification and localization of proteins or other biomolecules of a histologic tissue section comprises enzymatically digesting the biomolecules of two similar tissue sections while substantially preserving the biomolecule positions in the tissue sections. Next, a mass spectrometric image of the digest products in one of the tissue sections is acquired. Then, the digest products of the other tissue section are extracted and separated and the mass spectra and daughter ion spectra of all the digest products are acquired. A list of all identifiable biomolecules of the tissue section is created by comparing the mass spectra and daughter ion spectra with spectra in biomolecule structure databases or spectral libraries. Finally, the biomolecules in the list are assigned to digest products of the same mass in the mass spectra used to create the mass spectrometric image of the thin tissue section.


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