The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 05, 2019

Filed:

Dec. 05, 2011
Applicants:

Hiroaki Suga, Tokyo, JP;

Jumpei Morimoto, Tokyo, JP;

Inventors:

Hiroaki Suga, Tokyo, JP;

Jumpei Morimoto, Tokyo, JP;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C40B 50/06 (2006.01); B01J 19/00 (2006.01); C12N 15/10 (2006.01); C12N 15/67 (2006.01); C07K 7/08 (2006.01); C12Q 1/34 (2006.01); C07K 14/705 (2006.01); C40B 50/08 (2006.01);
U.S. Cl.
CPC ...
B01J 19/0046 (2013.01); C07K 7/08 (2013.01); C07K 14/705 (2013.01); C12N 15/1062 (2013.01); C12N 15/67 (2013.01); C12Q 1/34 (2013.01); B01J 2219/00725 (2013.01); C40B 50/06 (2013.01); C40B 50/08 (2013.01);
Abstract

Object is to provide a method of constructing a library of peptides having, at a desired position in the random sequence of each peptide, an amino acid having a portion capable of binding to a target. The invention provides a method of producing a library of peptides having, at a designated position in the random sequence of each peptide, a special amino acid having a portion capable of binding to a target, including (i) preparing a library of mRNAs having, in the mRNA sequence coding for a random amino acid sequence, a base sequence having an altered codon encoding the special amino acid, (ii) preparing an aminoacyl tRNA with the special amino acid linked to a tRNA encoded by the altered codon, and (iii) translating the mRNAs by using a cell-free translation system containing the aminoacyl tRNA to obtain a library of peptides having, in the random sequence thereof, a predetermined special amino acid.


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