The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 05, 2019

Filed:

Jul. 24, 2014
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

William L. Murphy, Waunakee, WI (US);

Ngoc Nhi Thi Le, Madison, WI (US);

Stefan Zorn, Madison, WI (US);

Michael P. Schwartz, Madison, WI (US);

Eric Huy Dang Nguyen, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/483 (2006.01); A61L 31/14 (2006.01); C12N 5/0735 (2010.01); G01N 33/50 (2006.01); A61K 47/42 (2017.01); A61L 29/16 (2006.01); B01J 19/00 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
A61L 31/145 (2013.01); A61K 47/42 (2013.01); A61L 29/16 (2013.01); C12N 5/0606 (2013.01); G01N 33/4833 (2013.01); G01N 33/5005 (2013.01); A61L 2300/80 (2013.01); B01J 19/0046 (2013.01); B01J 2219/00596 (2013.01); C12N 2533/20 (2013.01); C12N 2533/30 (2013.01); C12N 2537/10 (2013.01); G01N 21/6452 (2013.01); G01N 2610/00 (2013.01);
Abstract

Patterned hydrogel arrays and methods of preparing patterned hydrogel arrays are disclosed. Advantageously, the methods used to prepare the patterned hydrogel arrays allow for controlling individual hydrogel spot conditions such as hydrogel spot modulus, hydrogel spot ligand identity and hydrogel spot ligand density, which allows for preparing a wide range of hydrogel spots in a single array format. Patterned hydrogel arrays can also be formed to include hydrogel-free pools surrounded by hydrogel. Additionally, the patterned hydrogel arrays of the present disclosure support the culture of a range of cell types. The patterned hydrogel arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.


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