The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 29, 2019

Filed:

May. 07, 2014
Applicant:

Micronics, Inc., Redmond, WA (US);

Inventor:

Heather K. Bouzek, Seattle, WA (US);

Assignee:

MICRONICS, INC., Redmond, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12Q 1/70 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 15/1003 (2013.01); C12N 15/1006 (2013.01); C12Q 1/706 (2013.01); C12Q 1/707 (2013.01);
Abstract

The present invention provides a rapid and highly effective method for the preparation of biological samples for detection of both DNA and RNA target molecules. The method comprises treatment of the sample with a clay mineral followed by lysis of the sample with an alkaline solution. The method is particularly suited for preparing complex biological samples, such as blood or plasma, for the simultaneous detection of DNA and RNA targets. Samples prepared by the method of the invention may be directly used as targets in PCR amplification. The method of the invention may conveniently be coupled with further steps and devices to perform molecular analyses for diagnostic and other applications.


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