The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 29, 2019

Filed:

Jun. 03, 2014
Applicant:

The United States of America, As Represented BY the Secretary of Agriculture, Washington, DC (US);

Inventors:

Daniel Solaiman, Dresher, PA (US);

Richard D. Ashby, Glenside, PA (US);

Jonathan A. Zerkowski, Wayne, PA (US);

Nereus W. Gunther, Wyndmoor, PA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/44 (2006.01); C12P 7/64 (2006.01); C07K 14/21 (2006.01); C12N 9/10 (2006.01); C12P 19/18 (2006.01); C12N 15/64 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12P 19/44 (2013.01); C07K 14/21 (2013.01); C12N 9/1051 (2013.01); C12N 15/52 (2013.01); C12N 15/64 (2013.01); C12P 7/64 (2013.01); C12P 7/6409 (2013.01); C12P 19/18 (2013.01); C12Y 204/01 (2013.01);
Abstract

NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C-C) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C-C) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (RL) is described. Expression of two of these genes, i.e., rhlA and rhlB, together inNRRL B-30761 increases RL production by at least 10-fold. Also the generation of a recombinantNRRL B-30761 capable of synthesizing dirhamnose-lipid (RL) is described. Characterization of RL and RL produced by the recombinantNRRL B-30761 is also described.


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