The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 29, 2019
Filed:
Apr. 22, 2014
Kyushu University, National University Corporation, Fukuoka-shi, Fukuoka, JP;
Hiroshima University, Higashihiroshima-Shi, Hiroshima, JP;
Takashi Yamamoto, Higashihiroshima, JP;
Tetsushi Sakuma, Higashihiroshima, JP;
Takahiro Nakamura, Fukuoka, JP;
Yusuke Yagi, Fukuoka, JP;
Yasuyuki Okawa, Fukuoka, JP;
KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION, Fukuoka-Shi, JP;
HIROSHIMA UNIVERSITY, Higashihiroshima-Shi, JP;
Abstract
The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. 'ii' (−2) A. A. contained in L of the formula 1, the aforementioned object could be achieved.(Helix A)-X-(Helix B)-L  (Formula 1)