The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 22, 2019

Filed:

Jan. 11, 2017
Applicant:

The Johns Hopkins University, Baltimore, MD (US);

Inventors:

Chien-Fu Hung, Timonium, MD (US);

T. C. Wu, Stevenson, MD (US);

Assignee:

The Johns Hopkins University, Baltimore, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 39/00 (2006.01); C07K 14/77 (2006.01); C07K 16/30 (2006.01); A61K 31/7088 (2006.01); A61K 38/07 (2006.01); A61K 39/395 (2006.01); A61K 48/00 (2006.01); C07K 5/11 (2006.01); C07K 7/06 (2006.01); C07K 16/18 (2006.01);
U.S. Cl.
CPC ...
C07K 14/77 (2013.01); A61K 31/7088 (2013.01); A61K 38/07 (2013.01); A61K 39/0005 (2013.01); A61K 39/39558 (2013.01); A61K 48/00 (2013.01); C07K 5/1019 (2013.01); C07K 7/06 (2013.01); C07K 16/18 (2013.01); C07K 16/30 (2013.01); A61K 39/00 (2013.01); A61K 2039/505 (2013.01); A61K 2039/585 (2013.01); A61K 2039/6056 (2013.01); A61K 2039/627 (2013.01); C07K 2317/52 (2013.01); C07K 2317/622 (2013.01); C07K 2319/00 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01); C07K 2319/40 (2013.01); C07K 2319/50 (2013.01);
Abstract

The present invention provides an immunoconjugate having the formula:T-c-E-c-Fcor T-c-Fc-c-E;wherein, T is a single chain variable portion fragment of a monoclonal antibody (scFv) directed to a target protein, polypeptide, or fragment thereof, which is highly expressed on cancer cells; E is two or more foreign immunogenic CD8T cell antigenic epitopes; c is a peptide or polypeptide fragment thereof, capable of being cleaved by a specific protease; and Fc is two or more Fc portions of an IgG antibody. Nucleic acid sequences encoding the same and vectors containing said nucleic acid sequences are also provided. Methods of making the immunoconjugate, along with methods of making target cells susceptible to CTL mediated cell killing, and methods for treatment of cancers are also provided.


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