The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 08, 2019
Filed:
Jul. 30, 2015
Applicant:
Vanadis Diagnostics, Sollentuna, SE;
Inventors:
Assignee:
Vanadis Diagnostics, Sollentuna, SE;
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/683 (2018.01); C12Q 1/6858 (2018.01); C12Q 1/6883 (2018.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C12Q 1/683 (2013.01); C12Q 1/6858 (2013.01); C12Q 1/6883 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/158 (2013.01);
Abstract
A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.