The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 08, 2019
Filed:
Mar. 17, 2015
Applicant:
Shiga University of Medical Science, Otsu-shi, Shiga, JP;
Inventor:
Shin-ya Morita, Otsu, JP;
Assignee:
SHIGA UNIVERSITY OF MEDICAL SCIENCE, Otsu-shi, Shiga, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/28 (2006.01); C12Q 1/26 (2006.01); C12Q 1/32 (2006.01); C12Q 1/44 (2006.01); C12Q 1/48 (2006.01); G01N 21/64 (2006.01); G01N 33/92 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/28 (2013.01); C12Q 1/26 (2013.01); C12Q 1/32 (2013.01); C12Q 1/44 (2013.01); C12Q 1/485 (2013.01); C12Y 101/03021 (2013.01); C12Y 111/01007 (2013.01); C12Y 207/0103 (2013.01); C12Y 301/04004 (2013.01); G01N 21/6428 (2013.01); G01N 33/92 (2013.01); G01N 2333/902 (2013.01); G01N 2333/908 (2013.01); G01N 2333/916 (2013.01); G01N 2333/91215 (2013.01); G01N 2405/04 (2013.01);
Abstract
A method for quantifying cardiolipin in a sample, comprises the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprises phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.