The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 08, 2019

Filed:

Oct. 24, 2016
Applicant:

Institute of Biotechnology, Academy of Military Medical Science, Pla, Beijing, CN;

Inventors:

Wei Chen, Beijing, CN;

Shipo Wu, Beijing, CN;

Lihua Hou, Beijing, CN;

Xiaohong Song, Beijing, CN;

Jinlong Zhang, Beijing, CN;

Ling Fu, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 39/00 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01); C07K 14/005 (2006.01); A61K 48/00 (2006.01); A61K 39/12 (2006.01); C12N 15/861 (2006.01); A61P 31/14 (2006.01);
U.S. Cl.
CPC ...
A61K 39/12 (2013.01); A61P 31/14 (2018.01); C07K 14/005 (2013.01); C12N 15/86 (2013.01); C12N 15/861 (2013.01); A61K 2039/5254 (2013.01); A61K 2039/5256 (2013.01); C12N 2710/10041 (2013.01); C12N 2710/10062 (2013.01); C12N 2710/10343 (2013.01); C12N 2760/14122 (2013.01); C12N 2760/14134 (2013.01);
Abstract

Provided are an Ebola virus envelope glycoprotein (that is GP protein) codon optimized nucleotide sequence, a human replication deficient recombinant adenovirus capable of expressing the nucleotide sequence, and applications in preparing a vaccine for preventing Ebola virus diseases. The nucleotide sequence takes a replication deficient 5 type adenovirus that is lack of E1 and E3 in a combined mode as a vector. HEK293 cells that integrate adenovirus E1 genes serve as a packaging cell line, and carried protective antigenic genes are codon optimized Zaire type Ebola virus Makona strain envelope glycoprotein genes. After the envelope glycoprotein genes are optimized by codon, the expression level in transfection cells is obviously improved.


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