The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 01, 2019

Filed:

May. 26, 2016
Applicant:

Dako Denmark A/s, Glostrop, DK;

Inventors:

Jesper Lohse, Herlev, DK;

Kenneth Heesche Petersen, Smorum, DK;

Assignee:

DAKO DENMARK A/S, Glostrup, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); C12Q 1/28 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
G01N 33/581 (2013.01); C12Q 1/28 (2013.01); C12Q 1/6806 (2013.01); G01N 2333/908 (2013.01);
Abstract

The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein 'cross-linker') in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.


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