The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 01, 2019

Filed:

Mar. 23, 2016
Applicant:

Hoffmann-la Roche Inc., Littel Falls, NJ (US);

Inventors:

Kay-Gunnar Stubenrauch, Penzberg, DE;

Rudolf Vogel, Weilheim, DE;

Assignee:

F. HOFFMANN-LA ROCHE INC., Nutley, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/543 (2006.01); C07K 16/28 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54393 (2013.01); C07K 16/2866 (2013.01); G01N 33/6854 (2013.01); C07K 2317/24 (2013.01);
Abstract

Herein is reported an enzyme linked immunosorbent assay for the detection of anti-drug antibodies against a drug antibody in a sample comprising a capture drug antibody and a tracer drug antibody, wherein the capture drug antibody and the tracer drug antibody are employed in a concentration of 0.5 μg/ml or more, the sample is incubated simultaneously with the capture drug antibody and the tracer drug antibody for 1 to 24 hours, the capture drug antibody and the tracer drug antibody are derivatized via a single lysine residue, the sample comprises 10% serum, and oligomeric human IgG is added to the sample prior to the incubation with the capture drug antibody and the tracer drug antibody.


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