The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 01, 2019

Filed:

Nov. 03, 2014
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Daniel Irimia, Charlestown, MA (US);

Ki Wan Bong, Seoul, KR;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); G01N 33/543 (2006.01); B01L 3/00 (2006.01); G01N 33/569 (2006.01); G01N 33/574 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1459 (2013.01); B01L 3/502761 (2013.01); G01N 15/1429 (2013.01); G01N 15/1463 (2013.01); G01N 15/1484 (2013.01); G01N 33/54326 (2013.01); G01N 33/569 (2013.01); G01N 33/56961 (2013.01); G01N 33/56966 (2013.01); G01N 33/56972 (2013.01); G01N 33/574 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/149 (2013.01); G01N 2015/1488 (2013.01); G01N 2015/1497 (2013.01);
Abstract

Methods for sorting cells include: arranging microparticles into an array on a substrate in a microfluidic device, in which the microparticles each include multiple reference markers; introducing multiple cells to the array of microparticles under conditions that enable at least some of the cells to adhere to the microparticles; removing the microparticles, to which the cells are adhered, from the substrate; transferring the microparticles, to which the cells are adhered, to a detection region; and detecting, for each of two or more microparticles that pass through the detection region, a microparticle feature; and sorting the two or more microparticles based on the detected features, in which the detected features are related to a phenotype of the cells.


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