The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 01, 2019

Filed:

Feb. 21, 2008
Applicants:

Paul Czernichow, Paris, FR;

Raphaël Scharfmann, Paris, FR;

Philippe Ravassard, Paris, FR;

Inventors:

Paul Czernichow, Paris, FR;

Raphaël Scharfmann, Paris, FR;

Philippe Ravassard, Paris, FR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12N 5/09 (2010.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0676 (2013.01); C12N 5/0693 (2013.01); A61K 35/12 (2013.01); C12N 2500/90 (2013.01); C12N 2503/00 (2013.01); C12N 2503/02 (2013.01); C12N 2510/00 (2013.01); C12N 2510/04 (2013.01); C12N 2533/52 (2013.01); C12N 2533/90 (2013.01); G01N 2800/042 (2013.01);
Abstract

The present invention relates to a method for preparing commercial scale quantities of human functional Betacells and to the establishment of cell lines. It also relates to a method of diagnosis using Beta cell tumors or cells derived thereof. The method comprises sub-transplantation procedure to enrich the graft in proliferating Betacells, allowing to generate human Betacell lines. Such lines express little amount of insulin and have a gene expression profile that resembles to adult Betacells. In addition, the human Betacell lines are able to normalize glycemia of diabetic mice when transplanted, demonstrating their insulin secretion capabilities.


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